A. IFA of drug-selected 3D7 parasites harbouring the AMA_M3 construct. The transgenic PfAMA1/DIII-HA product detected by anti-HA mAb 3F10 (green) co-localises with the microneme protein EBA-175 (red) in mature segmented schizonts. The inset shows similar staining of a free merozoite, where some translocation of PfAMA1/DIIIHA onto the merozoite surface is detectable as is normal for endogenous PfAMA1. B. IFA of drug-selected 3D7 parasites harbouring the AMA_ins construct. The transgene product detected by anti-HA mAb 3F10 (green) co-localises correctly with the microneme protein EBA-175 (red) in mature segmented schizonts.

Olivieri A, Collins CR, Hackett F, Withers-Martinez C, Marshall J, Flynn HR, Skehel JM, Blackman MJ. Juxtamembrane shedding of Plasmodium falciparum AMA1 is sequence independent and essential, and helps evade invasion-inhibitory antibodies. PLoS Pathog. 2011 7(12):e1002448.