PfCENH3 localization in ring stage parasites. 3D7 wild-type parasites of ring stages were subjected to immunofluorescence assay with αPfCENH3. At this stage the PfCENH3 signal is substantially weaker and seldom concentrated to a single focus, suggesting that centromeres do not congregate into a single cluster at this stage. 3-6 distinct foci could typically be observed per nuclei potentially indicating sub-clustering of centromeres in ring stage nuclei.

Hoeijmakers WA, Flueck C, Françoijs KJ, Smits AH, Wetzel J, Volz JC, Cowman AF, Voss T, Stunnenberg HG, Bártfai R. Plasmodium falciparum centromeres display a unique epigenetic makeup and cluster prior to and during schizogony. Cell Microbiol. 2012 14(9):1391-401.