Transgenic expression of myc-tagged ETRAMP2 and ETRAMP4. Immunofluorescence analysis in acetone-fixed IRBCs: the tagged transgenes detected via the myc tag colocalize with the respective chromosomally derived ETRAMPs in a typical peripheral staining in the transfected parasites D10E2myc (C) and D10E4myc (D). C shows two IRBCs, each with a double infection of ring-stage parasites and D shows a double infection of trophozoites. Hoechst was used to stain the nuclei. Immunofluorescence analysis with an anti-myc serum showed a circular staining pattern in acetone-fixed D10E2myc and D10E4myc parasites (C and D), which is typical of the ETRAMP staining pattern seen with anti-ETRAMP serum in untransfected parasites. ETRAMPs are seen in focused areas of the PVM.

Spielmann T, Gardiner DL, Beck HP, Trenholme KR, Kemp DJ. Organization of ETRAMPs and EXP-1 at the parasite-host cell interface of malaria parasites. Mol Microbiol. 2006 59:779-94.