Schizont and merozoite culture was incubated overnight with 0.1µM cytochalasin D to inhibit invasion. Thin smeared on a glass slide, fixed in methanol, stained with Rabbit anti-PfRh5 polyclonal serum 1:200 and secondary Alexa-Flor488 anti-Rabbit. Dual colour fluorescence images were captured using a Carl Zeiss Axioskop 2 microscope at 100x using Oil imersion. Nucleus is marked with DAPI. Scale bar indicate 1µM. PfRh5 localizes to the rhoptries (as shown by immuno-EM).

Baum J, Chen L, Healer J, Lopaticki S, Boyle M, Triglia T, Ehlgen F, Ralph SA, Beeson JG, Cowman AF. Reticulocyte-binding protein homologue 5 - An essential adhesin involved in invasion of human erythrocytes by Plasmodium falciparum. Int J Parasitol. 2008 39(3):371-80 . Copyright Elsevier 2009.