Subcellular localization of PfUT by immunoelectron microscopy. The upper panel shows a repre-sentative micrograph of a P. falciparum-infected erythrocyte preserved by high-pressure freezing and freeze-substitution, and immunolabelled with a rabbit antiserum specific to the N-terminal domain of PfUT (1:100) coupled to 10 nm protein A colloidal gold. The lower panel shows the surface rendered view of the micrograph, with red dots representing gold grains. Insert: Magnification of boxed section in micrograph. Arrowheads point towards gold label. n, nucleus; fv, food vacuole. Scale bar in D and E, 500 nm. Gold grains were significantly more present in areas of ER/Golgi complex (ER) than in other subcellular compartments.

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