Immunolocalization of a constitutively active GTPase mutant of Rab5a. The immunoelectron micrograph shows a parasite expressing GFP-Rab5a (Q102L) - a constitutively active mutation. The section was probed with gold-conjugated anti-GFP antisera. Note that numerous gold particles decorate the hemoglobin-containing vacuole (HV) (Inset). The food vacuole (FV) and the nucleus (N) are indicated. (Scale bar, 1 µm.) Because this is merely 2D immuno-TEM, we cannot precisely determine whether the parasite is a trophozoite or a schizont.

Elliott DA, McIntosh MT, Hosgood HD 3rd, Chen S, Zhang G, Baevova P, Joiner KA. Four distinct pathways of hemoglobin uptake in the malaria parasite Plasmodium falciparum. Proc Natl Acad Sci U S A. 2008 105(7):2463-8. PMID: