Genome-wide binding profile of PfTRZ. a,b, Localization of endogenous PfTRZ-GFP (a) and PfTRZ-3×HA (b), detected by IFA in three different IDC stages. h.p.i., hours post-invasion. Scale bars, 5 μm. Results are representative of three biological replicate experiments. c, Live imaging of endogenous PfTRZ-GFP. Scale bar, 5 μm. Results are representative of three biological replicate experiments. d, Co-localization of PfTRZ-3×HA and PfHP1 by confocal laser-scanning microscopy. The Pearson co-localization map visualizes overlapping signals (right). Scale bar, 2 μm. PfTRZ-GFP and PfTRZ-3×HA localized to distinct foci at the nuclear periphery throughout the intra-erythrocytic developmental cell cycle. The number of PfTRZ foci and total PfTRZ expression levels increased in schizonts (a,b). Confocal laser-scanning microscopy confirmed the exclusive co-localization of PfTRZ with PfHP1 at chromosome-end clusters.

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