surface-related antigen (PfSRA) antibodies recognize the native parasite protein. A, Malaria-infected patient plasma from 3

endemic sites (Kintampo, Navrongo, and Accra) in Ghana detected PfSRA peptides by immuno-dot blotting. B, Colocalization of α-PfSRA P3 human antibody (green; 1:50) with α-PfMSP119 mouse antibody (red; 1:100) showed that PfSRA is localized on the merozoite surface. Nuclei were stained with 4′,6-diamidino-2-phenylindole (blue). Secondary antibodies used were fluorescein isothiocyanate-conjugated goat α-human immunoglobulin G (IgG; 1:200) and Alexa 568–conjugated goat α-mouse IgG (1:200; Life Technologies). Abbreviations: DAPI, 4′,6-diamidino-2-phenylindole; PfSRA, Plasmodium falciparum surface-related antigen.

Amlabu E, Mensah-Brown H, Nyarko PB, Akuh OA, Opoku G, Ilani P, Oyagbenro R, Asiedu K, Aniweh Y, Awandare GA. Functional Characterization of Plasmodium falciparum Surface Related Antigen (PfSRA) as a Potential Blood-Stage Vaccine Target. J Infect Dis. 2018 Apr 18. [Epub ahead of print]