Upper panel: Distribution of PfVPS35 upon compound 5f treatment in P. falciparum K1 strain where chloroquine was ineffective. Parasite was treated with CQ and compound 5f as described in Materials and Methods followed by fixing and immunostaining of PfRab7 and PfVPS35. Scale bar in each picture indicates 5 μm. Lower panel: The localization of PfRab7 and PfVPS35 in presence of CID 1067700 (10 μM). Scale bar in each picture indicates 5 μm. CID 1067700 affected the localization pattern of PfVPS35 that resulted in diffused signals. Data demonstrated that the inhibition of PfRab7 resulted in the disassembly of retromer complex. This further indicated the possible role of PfRab7 as a regulator retromer complex in P. falciparum.

Siddiqui AA, Saha D, Iqbal MS, Saha SJ, Sarkar S, Banerjee C, Nag S, Mazumder S, De R, Pramanik S, Debsharma S, Bandyopadhyay U. Rab7 of Plasmodium falciparum is involved in its retromer complex assembly near the digestive vacuole. Biochim Biophys Acta Gen Subj. 2020 Jun 5:129656.