IFA of gametocyte-infected erythroblasts on methanol-fixed bone marrow smears from a P.falciparum-infected patient. Gametocytes were stained with anti-Pf11-1 antibody (green) and erythroblasts with anti-glycophorin A (GPA) labelling the erythroid membrane (red). DNA is stained with Hoechst 33342 (blue). i, ii: stage I-II gametocyte inside erythroblast ; iii, iv: stage III-IV gametocyte inside erythroblast ; v: stage I-II gametocyte inside reticulocyte or erythrocyte; vi: stage III-IV gametocyte inside reticulocyte or erythrocyte ; vii: stage V gametocyte inside reticulocyte or erythrocyte. Gametocyte stages were evaluated according to the size and the shape of P11-1-positive cells. Bars represent 5 μm.

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