Immunodetection of SR10 in the P. falciparum blood stages. C. Subcellular localization of SR10 in blood stage parasites. WT trophozoites (TZ), schizonts (SZ), gametocytes (GCII-GCV) and activated gametocytes (aGC; at 30 min p.a.) were immunolabelled with mouse anti-SR10 antisera (green). The asexual blood stages were counterlabelled with rabbit anti-MSP1 antibody; the gametocytes were counterlabelled with rabbit anti-Pfs230 antisera and the activated gametocytes with anti-Pfs25 antibody (red). The parasite nuclei were highlighted by Hoechst33342 nuclear stain (blue). Bar, 5 μm. E. Subcellular localization of SR10-HA in blood stage parasites. Trophozoites (TZ), schizonts

(SZ), gametocytes (GCII-GCV) and activated gametocytes (aGC; at 30 min p.a.) of the SR10-HA line were immunolabelled with rabbit anti-HA antibody (green). The asexual blood stages were counterlabelled with mouse anti-SR10 antisera; the gametocytes were counterlabelled with mouse mouse anti-Pfs230 antisera (red). The parasite nuclei were highlighted by Hoechst33342

nuclear stain (blue). Bar, 5 μm.

Njila Tchoufack EJ, Hahnfeld L, Pitschelatow G, Bennink S, Pradel G. The endoplasmic reticulum-resident serpentine receptor SR10 has important functions for asexual and sexual blood stage development of Plasmodium falciparum. Mol Biochem Parasitol. 2020 Sep 2:111315.