Targeting PI(3)P synthesis disrupts the subcellular localization of a DV-resident protein plasmepsin II under heat stress. Representative images from live cell confocal microscopy are shown. GFP, green fluorescent protein-tagged plasmepsin II; DIC, differential interference contrast. Scale bar, 2 mm. we examined if chemically inhibiting PI(3)P production affects DV membrane stability and morphology. However, Wortmannin treatment resulted in a high background signal and PM2GT mis-localization even at 37°C (no heat shock), making it difficult to identify heat-dependent changes. In contrast, LY294002-treated parasites showed normal DV morphology and PM2GT localization at 37°C, with DV MFIs comparable to the DMSO control.

Lu KY, Pasaje CFA, Srivastava T, Loiselle DR, Niles JC, Derbyshire E. Phosphatidylinositol 3-phosphate and Hsp70 protect Plasmodium falciparum from heat-induced cell death. Elife. 2020 Sep 25;9:e56773. PMID: 32975513.