The charged region (CR) of PfEH1 is required for export, and the a/b-hydrolase region is required for cytoskeletal tethering. Schematic representing the PfEH1 truncation(Trunc) constructs. Each construct contains the N-terminal signal sequence and PEXEL motif, as well as a linker region (L) before the reporter GFP. SP, signal peptide. ABH, a/b-hydrolase. (Right) Images from IFA of paraformaldehyde/glutaraldehyde-fixed, trophozoite/early schizont-stage parasites expressing the truncated PfEH1 under control of the HSP86 promoter. The nuclei were stained with DAPI (blue). Bars = 5 mm. DIC, differential interference contrast.

Spillman NJ, Dalmia VK, Goldberg DE. Exported Epoxide Hydrolases Modulate Erythrocyte Vasoactive Lipids during Plasmodium falciparum Infection. MBio. 2016 Oct 18;7(5). pii: e01538-16. PMID: 27795395