PF3D7_0512900 AKAP-like protein AKAL

PfAKAL subcellular localizations in asexual and sexual P. falciparum blood stages. Images with PfAKAL localization (green), Hoechst DNA staining (blue), differential interference contrast (DIC) were taken on rings a trophozoites, b schizonts, c as well as on stage II, d stage III, e stage IV, f and stage V, g gametocytes, infected erythrocytes. “Bright orange” on DIC images indicates haemozoin crystals. The bars represent 2 μm. In asexual parasites PfAKAL was undetectable in ring stages (a), with expression predominantly in late trophozoites and schizonts. Interestingly, a diffuse subcellular localization of PfAKAL was observed in the parasite cytoplasm and in the nucleus in trophozoite stages, whereas it was mostly nuclear in schizonts (b, c). In sexual stages, PfAKAL was also detected in the cytoplasm of the parasite, although it mainly accumulates around the nucleus.

Bandje K, Naissant B, Bigey P, Lohezic M, Vayssières M, Blaud M, Kermasson L, Lopez-Rubio JJ, Langsley G, Lavazec C, Deloron P, Merckx A. Characterization of an A-kinase anchoring protein-like suggests an alternative way of PKA anchoring in Plasmodium falciparum. Malar J. 2016 15(1):248.

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