Localization of PfeIF2b. Immunolocalization assays.Asynchronous cultures of PfeIF2b-Hatag recombinant strain of P.falciparum 3D7were fixed with formalin and paraffin embedded. Sections were incubated with an anti-HAtag(biotin) antibody recognized by a streptavidine-Alexafluor488-labeled conjugated added with DAPI to label nuclei. Fluorescence staining was analyzed using a Zeiss LSM880 confocal microscope.The merged image of the doubl estained (PfeIF2b-HAtag,DAPI) and differential interference contrast (DIC )images are also presented. Immunofluorescence assays revealed a cytoplasmic localization of PfeIF2b in the trophozoite (panel1), young schizont (panel2), or mature schizont (panel3) stages. Note that no staining was observed in ring-stage. No staining was observed when the primary antibody (anti-HA) was omitted (not shown). Bar = 5 μm.

Tellier G, Lenne A, Cailliau-Maggio K, Cabezas-Cruz A, Valdés JJ, Martoriati A, Aliouat el M, Gosset P, Delaire B, Fréville A, Pierrot C, Khalife J. Identification of Plasmodium falciparum Translation Initiation eIF2β Subunit: Direct Interaction with Protein Phosphatase Type 1. Front Microbiol. 2016 26;7:777.