Blood stage-specific PfCLK expression and co-localization with the parasite nucleus. Indirect immunofluorescence assay using anti-PfCLK-1antibody showed distinct punctate protein expression in association with the nucleus of trophozoites (TZ) and schizonts (SZ). In gametocytes (GC) PfCLK-1expression was not restricted to the nucleus, but also present in the cytoplasm. PfCLK-2 was present in nucleus and cytoplasm of asexual parasites and gametocytes. In gametocytes, an additional labelling of the erythrocyte hem in a punctate pattern was observed. Kinase labelling was visualized with AlexaFluor488 secondary antibody (green), nuclei were highlighted by Hoechst staining (blue), red blood cells (RBC) were counterstained with Evans Blue (red). Bar, 5 μm.

Agarwal S, Kern S, Halbert J, Przyborski JM, Baumeister S, Dandekar T, Doerig C, Pradel G. Two nucleus-localized CDK-like kinases with crucial roles for malaria parasite erythrocytic replication are involved in phosphorylation of splicing factor. J Cell Biochem. 2011 112(5):1295-310