PfMC-2TM is exported via classical secretory pathway during the ring stage of Plasmodium falciparum (3D7) strain. Immediately after merozoite invasion, 3D7 ring stage parasites were treated with BFA for 16 h and timepoints were collected at 4, 8 and 16 h, respectively. The cells after respective timepoints were washed and recovered. (A). Monoclonal antibodies (mAb) SP1C1 specific for PfMC-2TM, anti-SERA1 specific for the parasitophorous vacuole protein (SERA1), and anti-RhopH3 (green) were used. (B) Antibodies SP1C1 specific for PfMC-2TM and antibodies specific for the C and N termini of PfMC-2TM were used. (C) Monoclonal antibody SP1C1 (red) specific for PfMC-2TM and antibodies specific for the MC resident proteins REX1 (green). MAHRP1 (green). (D) SP1C1 and antibody SP1A6 against the MC protein Pf130 were also incubated with anti-PfERD2. Briefly, PfMC-2TM (red), SERA1 (green), RhopH3 (green), REX1 (green) and PfERD2 (green) were visible inside the parasite in close proximity to the nucleus in Brefeldin-A treated cells. Removal of drug pressure shows translocation of PfMC-2TM into the cytoplasm and association with the MCs in the erythrocyte cytoplasm. Yadavalli R, Peterson JW, Drazba JA, Sam-Yellowe TY. Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer's Clefts. Pathogens. 2021 10(4):431.