The localization of PfMC-2TM to Maurer’s clefts immediately after parasite invasion of red blood cell. (A) Stage specific expression and localization of PfMC-2TM in P. falciparum 3D7 infected erythrocytes was visualized using SP1C1 (mAb) and co-localized with REX1 (green) specific antibodies. Infected red blood cells were percoll synchronized and collected 4, 8, 12, 16, 30, 42 and 51 h post invasion of merozoites. Expression of PfMC-2TM (red) and co-localization with REX1 (green). (B) Stage specific expression and localization of RhopH3 and SERA 1 using Anti-SERA1 and anti-RhopH3 antisera were used for endogenous expression controls. (C) Negative control normal rabbit serum and normal mouse serum do not react with parasite proteins and uninfected red blood cells. Nuclei are stained with DAPI. Yadavalli R, Peterson JW, Drazba JA, Sam-Yellowe TY. Trafficking and Association of Plasmodium falciparum MC-2TM with the Maurer's Clefts. Pathogens. 2021 5;10(4):431