C-terminally tagged PfAlba1-Ty1 was successfully expressed from an episome in P. falciparum asexual blood stages. Immunofluorescence assays were used to determine the localization of PfAlba1-Ty1 in trophozoite (T) and schizont (S) stages of PfAlba1-Ty1 parasites. Empty vector transfectants served as a negative control. Antibodies used included mouse anti-Ty1 (green) or anti-PfAlba1 (red). Nuclei were labeled with DAPI (blue). Scale bar represents 1 μM. PfAlba1-Ty1 was predominantly cytoplasmic as was described for native PfAlba1.

Vembar SS, Macpherson CR, Sismeiro O, Coppée JY, Scherf A. The PfAlba1 RNA-binding protein is an important regulator of translational timing in Plasmodium falciparum blood stages. Genome Biol. 2015 16:212. PMID: 26415947