(A and B) Double-labeling immunofluorescence assays using rabbit anti-PfPol I and rat anti-PfNop1 (A) or anti-PfNop5 (B) antibodies, showing localization of the nucleolus at periphery of nucleus, forming a “hat-like” structure. (C) RNA FISH (using a 18S universal probe that hybridizes to all rRNAs produced in the parasite, green) combined with immunofluorescence microscopy (using the anti-PfPolI antibody, red), demonstrating that the region detected by the antibodies corresponds to the nucleolus of the parasite. In all images, parasites are in the ring stage, and the nuclear DNA is stained with DAPI (blue). (Scale bars, 1 μm.)

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