Blood stages of the PfSAMS-pfama1-GFP-BirA line and gametocytes of the PfSAMS-pffnpa-GFP-BirA line were treated with 50 μM biotin for 24 h. WT NF54 was used as a negative control. Immunofluorescence assays were employed, using mouse antibodies directed against Pf39 to highlight trophozoites (TZ), and schizonts (SZ) and rabbit antisera directed against Pfs230 to highlight immature (imGC) and mature (mGC) gametocytes (green). Biotinylated proteins were immunolabeled using fluorophore-conjugated streptavidin (red). The nuclei were labeled with Hoechst 33342 nuclear stain (blue). Bar; 5 μm.

Musabyimana JP, Distler U, Sassmannshausen J, Berks C, Manti J, Bennink S, Blaschke L, Burda PC, Flammersfeld A, Tenzer S, Ngwa CJ, Pradel G. Plasmodium falciparum S-Adenosylmethionine Synthetase Is Essential for Parasite Survival through a Complex Interaction Network with Cytoplasmic and Nuclear Proteins. Microorganisms. 2022 Jul 14;10(7):1419. doi: 10.3390/microorganisms10071419.