Analysis of ER retrieval strength compared to mutant transit peptides. A) Imaging of parasites expressing the K22E mutant form of ACP apicoplast leader sequence (BTSK22E) fused to GFP_KDEL (left panel) or GFP_SDEL (right panel). Upper panel: live-cell imaging; middle and lower panels: immunofluorescence assay using anti-PfACP or anti-PfERC. B) Imaging of parasites expressing the K18E mutant form of ACP apicoplast leader sequence (BTSK18E) fused to GFP_KDEL (left panel) or GFP_SDEL (right panel). Upper panel: live-cell imaging; middle and lower panels: immunofluorescence assay using anti-PfACP or anti-PfERC. Scale bar: 3 μm.
Heiny SR, Pautz S, Recker M, Przyborski JM. Protein Traffic to the Plasmodium falciparum Apicoplast: Evidence for a Sorting Branch Point at the Golgi. Traffic. 2014 5(12):1290-304.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0208500 | acyl carrier protein |