PF3D7_1116700 dipeptidyl aminopeptidase 1 cathepsin C, homolog

Deconvolving micrographs of Plasmodium-infected RBCs show that GSL-II, which binds to Plasmodium N-glycans, colocalizes with rhoptries (row A) and the ER (row B) but does not colocalize with apicoplasts (row C), the parasitophorous vacuole (row D), or the food vacuole (row E). In each case GSL-II is labeled red with Alexafluor 594, while antibodies labeled green with Alexafluor 488 are against RhopH3 (rhoptries), Pf39 (ER), and food vacuole (DPAP1) (14, 30, 51). Alternatively, GFP is targeted to apicoplasts (ACPleader-GFP) and the parasitophorous vacuole (ACPsignal-GFP) (55). Control experiments with anti-GFP antibodies show that both apicoplasts and parasitophorous vacuoles are accessible to exogenous probes.

Bushkin GG, Ratner DM, Cui J, Banerjee S, Duraisingh MT, Jennings CV, DvorinJD, Gubbels MJ, Robertson SD, Steffen M, O'Keefe BR, Robbins PW, Samuelson J. Suggestive evidence for Darwinian Selection against asparagine-linked glycans of Plasmodium falciparum and Toxoplasma gondii. Eukaryot Cell. 2010 9(2):228-41. PMID: 19783771

Other associated proteins

PFID Formal Annotation
PF3D7_0208500 acyl carrier protein
PF3D7_0905400 high molecular weight rhoptry protein 3
PF3D7_1108600 endoplasmic reticulum-resident calcium binding protein
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