PF3D7_1411400 plastid replication-repair enzyme

Subcellular localization of PfPREX. Malaria parasites were doubly transfected with pSSPF2/PfPREX-GFP and pSSPF2/PfACP-DsRED (A and B),or with pSSPF2/Pf PREX-GFP alone (C and D). The mitochondrion of the pSSPF2/Pf PREX-GFP single transfectant parasite (C and D) was stained with MitoTracker Red CM-H2

XRos (MTR). Panels in each row represent: green signal of GFP (far left); red signal of DsRED specifically localizing to the apicoplast (A and B) or MTR to the mitochondrion (C and D) (second from the left); merge of the green and red signals as well as the blue signal of Hoechst33342 indicating the position of the parasite nucleus (second from the right); overlay of the merged image with the phase contrast image (far right). (A) Early trophozoite; (B) Schizont; (C) Early trophozoite; (D) Schizont. Scale bar: 5mm.

Seow F, Sato S, Janssen CS, Riehle MO, Mukhopadhyay A, Phillips RS, Wilson RJ, Barrett MP. The plastidic DNA replication enzyme complex of Plasmodium falciparum. Mol Biochem Parasitol. 2005 141(2):145-153. PMID: 15926203

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