Inhibition of Cct4, Mcm2, or Rvb2 ATPase Activity Affects Stress Granule Assembly/Disassembly (A) Wild-type or cct4-1 cells carrying Pab1-GFP imaged after shifting to 37C (60 min) before NaN3 stress, after NaN3 treatment for 10 min, after NaN3 treatment for 20 min, or with 1 M KCl for 30 min. (B) Wild-type or cct4-1 cells carrying Pab1-GFP imaged prior to stress, after 30 min of NaN3, and after 60 min of recovery at 37C. Graph shows mean from three experiments ± SD. (C) Wild-type and mcm2-1 yeast cells with Pab1GFPprior to stress, after stress (30 min), and upon recovery from stress. Graph shows mean from three experiments ± SD. (D) Same as (C) but for rvb2-1 and the corresponding wild-type yeast cells. *p < 0.05; **p < 0.01; ***p < 0.0001. Scale bars, 2 mm. Jain S, Wheeler JR, Walters RW, Agrawal A, Barsic A, Parker R. ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure. Cell. 2016 164(3):487-98. PMID: 26777405;