Confocal micrographs showing the subcellular location of GFP-tagged PfPanK1 in 3D7 strain parasites harbouring the Pfpank1- pGlux-1 episomal plasmid. From left to right: Brightfield, GFP-fluorescence, DAPI- (fixed cells; top) or Hoechst 33258- (live cells; bottom) fluorescence, and merged images of erythrocytes infected with trophozoite-stage P. falciparum parasites expressing PfPanK1-GFP. Arrows indicate the plasma membranes of either the erythrocytes (black) or the parasites (white). Scale bar represents 5 μm.
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