Assessment of PfTPxGl47-EGFP topology in the membrane. (D) Schematic representation of the possible outcomes of split-GFP complementation in the ER and the cytosol. Split-GFP complementation in parasites expressing PfTPxGl47-S11, and either (E) ER luminal GFPS1-10 or (F) cytosolic GFPS1-10. Scale bar = 5 lm. The localizations of both ERS1–10 and CyS1–10 were confirmed by immunostaining with polyclonal anti-GFP antibodies. The distinct perinuclear fluorescence staining pattern observed for ERS1–10 and the diffused fluorescence observed throughout the cell for CyS1–10 are patterns that are consistent with the expected ER and cytosolic localizations of these proteins. Green fluorescence was observed in the ER lumen only in parasite lines expressing PfTPxGl47-S11 that had been transfected with ERS1–10 (E), but not in the CyS1–10 transfectants (F).
Narayan A, Mastud P, Thakur V, Rathod PK, Mohmmed A, Patankar S. Heterologous expression in Toxoplasma gondii reveals a topogenic signal anchor in a Plasmodium apicoplast protein. FEBS Open Bio. 2018 8(11):1746-1762. eCollection
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1212000 | glutathione peroxidase-like thioredoxin peroxidase |