SMC3 is part of a conserved cohesin complex in P. falciparum. A Cohesin complex subunits annotated in P. falciparum. The hinge and ATPase domains of SMC1 and SMC3 are indicated. E. Western blot analysis with an anti-HA antibody or in-house-generated anti-SMC3 antibody (indicated to the left of each blot) of nuclear extracts from synchronous SMC1-3HA-dd (D) or STAG-3HA-dd (E) late-stage parasites before (input, “In”) and after (flow-through, “FT”) immunoprecipitation with an antibody against HA (“a-HA IP”). An antibody against histone H3 is used as a control. Immunoprecipitation with IgG (“IgG IP”) was used as a control in the right panel of (E). Molecular weights are shown to the right. SMC1-3HA-dd has a predicted molecular weight of approximately 230 kDa and STAG-3HA-dd has a predicted molecular weight of approximately 298 kDa (of which 3.3 and 11.9 kDa correspond to the 3HA tag and the ddFKBP, respectively).