Upper panel: Specific labeling with mouse anti-Vsp4 of the cytosol. ER, endoplasmic reticulum; N, nucleus; Pf, Plasmodium falciparum; DV, digestive vacuole. Bar, 0.15 mm. Lower panel: Mutant GFP-PfVps4 localizes to punctuate structures when transiently expressed in P. falciparum. (B) Pattern of GFP fluorescence in stable line of P. falciparum expressing GFP-PfVps4-WT. Corresponding phase contrast images are illustrated. (C) Pattern of GFP fluorescence in P. falciparum transiently transfected with GFP-PfVps4-E214Q, mutated to block ATP hydrolysis. Arrows indicate the punctuate structures.
Yang M, Coppens I, Wormsley S, Baevova P, Hoppe HC, Joiner KA. The Plasmodium falciparum Vps4 homolog mediates multivesicular body formation. J Cell Sci. 2004 117:3831-8.