IFA of merozoites stained with a mAb specific for pfGAPDH, pfAldolase and RAP-1. Row 1 demonstrates merozoites stained with anti-pfGAPDH mAb (green, column I), anti-pfAldolase mAb (red, column II) and the overlay of both images (column III). Row 2 shows merozoites stained with anti-pfGAPDH mAb (green, column I) and anti-RAP-1 mAb 5-2 (red, column II) and the overlay of both images (column III). pfAldolase and pfGAPDH were colocalized in the early stages of parasite development since both, the green and the red signals, were super-imposed resulting in orange signals. At 40 h post synchronization the green signal of the anti-pfGAPDH staining was largely confined to the periphery of the schizont, while the red signal of the anti-pfAldolase staining remained distributed equally throughout the schizont. pfGAPDH and pfAldolase were preferentially localized at opposite cell poles. pfGAPDH was preferentially co-localized with RAP-1 which is associated with the apical complex of the merozoites.
Daubenberger CA, Tisdale EJ, Curcic M, Diaz D, Silvie O, Mazier D, Eling W, Bohrmann B, Matile H, Pluschke G. The N'-terminal domain of glyceraldehyde-3-phosphate dehydrogenase of the apicomplexan Plasmodium falciparum mediates GTPase Rab2-dependent recruitment to membranes. Biol Chem. 2003 384:1227-37. Copyright Walter de Gruyter GmbH 2009
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1410400 | rhoptry-associated protein 1 |
PF3D7_1444800 | fructose-bisphosphate aldolase |