confocal microscopy was performed to localize PfMORC protein in 3D7morc-GFP-infected erythrocytes. Asexual parasites at ring (6 h.p.i.), early-trophozoite (24 h.p.i.), late-trophozoite (34 h.p.i.) and schizont (44 h.p.i.) stage were used to localize the PfMORC. Parasites were also stained with the nuclear stain DAPI. The colocalization of GFP with the DAPI signal confirms that PfMORC localizes in the nucleus of the asexual developmental stages.
Singh MK, Tessarin-Almeida G, Dias BKM, Pereira PS, Costa F, Przyborski JM, Garcia CRS. A nuclear protein, PfMORC confers melatonin dependent synchrony of the human malaria parasite P. falciparum in the asexual stage. Sci Rep. 2021 11(1):2057.