PF3D7_1468100 MORC family protein

PfMORC-HA is associated with heterochromatin. (a) Illustration of the PfMORC containing domains including Kelch type b propeller and ATPase domains using InterProScan. (b) Design strategy applied for PfMORC C-terminal HA tagging. PCR amplification of the genomic C-terminus end of Pfmorc region extending towards the 3’UTR (F1) as well as extension from the C-terminus towards the HA flanking sequence (F2) verifies correct insertion site. NF54 genomic DNA was used as negative control. (c) IFA experiment: PfMORC foci expressing co-localization with H3K9me3 marks (red). Cell nuclei are stained with DAPI (blue). BF: brightfield (d) Protein immunoprecipitation: Significance plot representing PfMORC interactome recovered through immunoprecipitation followed by mass spectrometry (IP-MS). Graph list MORC (orange) bindings partners of highest affinity associated with TF regulation and chromatin remodelers, erasers, and writers (purple). Proteins enriched in the PfMORC-HA samples compared with controls were filtered with log2 FC ≥ 2 and Z statistic > 5. (e) Gene ontology enrichment analysis of the significantly enriched proteins. The top 2 terms of Cellular Component (top) and top 3 terms of Molecular Function (bottom) are represented as −log10 (adjusted P-value) (Fisher's exact test with Bonferroni adjustment). Chahine Z, Gupta M, Lenz T, Hollin T, Abel S, Banks C, Saraf A, Prudhomme J, Florens L, Le Roch KG. PfMORC protein regulates chromatin accessibility and transcriptional repression in the human malaria parasite, P. falciparum. bioRxiv [Preprint]. 2023. PMID: 37745554;

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