Localization of candidate apicoplast proteins identified by BioID. Fixed-cell imaging of GFP-tagged candidates in parasites stained with an antibody raised against the apicoplast marker ACP. ROM7±GFP-expressing parasites were also stained with anti-GFP antibody due to low signal from intrinsic GFP fluorescence in fixed cells. Arrowheads indicate regions where PF3D7_0721100-GFP puncta appear adjacent to as opposed to colocalizing with ACP. Scale bars, 5 μm. ACP, acyl carrier protein; BioID, proximity-dependent biotin identification; GFP, green fluorescent protein; IPP, isopentenyl pyrophosphate; ROM, rhomboid protease homolog.

Boucher MJ, Ghosh S, Zhang L, Lal A, Jang SW, Ju A, Zhang S, Wang X, Ralph SA, Zou J, Elias JE, Yeh E. Integrative proteomics and bioinformatic prediction enable a high-confidence apicoplast proteome in malaria parasites. PLoS Biol.

2018 16(9):e2005895.