Upper 2 panels: Colocalization of gene expression sites with nuclear pores. (A) Combination of anti-PfNup116 immuno-fluorescence staining with RNA-FISH using a probe against the active var gene expressed in 3D7-G7 clone (PF3D7_0412700). Nucleus stained by DAPI. Bar, 1 μm.(B) As in (A) with FISH probe against 18S ribosomal RNA. (C) Fraction of cells with an overlap of FISH and nuclear pore foci. Lower panel: Colocalization of PfSet10-HA with nuclear pores. Combination of anti-PfNup116 and anti-HA immunofluorescence staining on early trophozoite stage parasite expressing recombinant PfSet10-HA. Nucleus stained by DAPI. Bar, 1 μm.

Guizetti J, Martins RM, Guadagnini S, Claes A, Scherf A. Nuclear pores and perinuclear expression sites of var and rDNA genes correspond to physically distinct regions in Plasmodium falciparum. Eukaryot Cell. 2013 12(5):697-702