3D7 parasites were prepared by U-ExM, stained with NHS ester (greyscale), BODIPY TRc (white), SYTOX (cyan) and anti-centrin (outer centriolar plaque (CP); magenta) antibodies and imaged using Airyscan microscopy.. (a) Images of whole parasites throughout asexual blood-stage development. (b) Whole parasite panel (left) followed by individual centriolar plaque or centriolar plaque pair zooms following our proposed timeline of events in centriolar plaque biogenesis, dynamics, and disassembly. Yellow line = cytoplasmic extensions, blue line = nuclear envelope, green line = parasite plasma membrane. Images are maximum-intensity projections, number on image = Z-axis thickness of projection in μm. White scale bars = 2 μm, yellow scale bars = 500 nm.
Stürmer VS, Stopper S, Binder P, Klemmer A, Lichti NP, Becker NB, Guizetti J. Progeny counter mechanism in malaria parasites is linked to extracellular resources. PLoS Pathog. 2023 19(12):e1011807. PMID: 38051755