The interaction between UBAP2L and G3BP1 is required for SG assembly. a List of proteins identified by mass spectrometry analysis. c HeLa cells were lysed and immunoprecipitated with either anti-rabbit IgG or anti-UBAP2L antibodies. The immunoprecipitates were blotted with anti-UBAP2L, anti-G3BP1 or anti-FXR2 antibodies. d Schematic representation of UBAP2L deletion constructs (N.D. (not detected)). e HeLa cells that constitutively expressed each construct were treated with 0.5 mM arsenite for 30 min and immunostained for TIAR and FLAG (scale bar = 10 μm). f HeLa cells that constitutively expressed each siRNA resistant cDNA were depleted of endogenous UBAP2L by UBAP2L siRNA #2. The cells were lysed and immunoblotted with anti-FLAG and anti-UBAP2L antibodies. g Cells that constitutively expressed each constructs were depleted of UBAP2L by siRNA and immunostained for FLAG and G3BP1 (scale bar = 10 μm). h The number of G3BP1-positive SGs per cell is presented in the graphs (n = 3 (total cells = 15), **P < 0.01, *P < 0.05). i The number of TIAR-positive SGs per cell is presented in the graphs (n = 3 (total cells = 15), **P < 0.01, *P < 0.05).
Asano-Inami E, Yokoi A, Sugiyama M, Hyodo T, Hamaguchi T, Kajiyama H. The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formation. Commun Biol. 2023 6(1):415. PMID: 37059803