PfVIT and PfE140 are important for P. falciparum growth and may be involved in intracellular iron homeostasis. (A) Representative erythrocytes infected with P. falciparum 3D7 parasites that endogenously express a truncated version of PfVIT or PfZIPCO tagged with GFP (green). (B) Growth rates of knockout parasite lines generated. (C) Representative live 3D7 parasites endogenously expressing PfE140-GFP whose green fluorescence was reduced by glmS-mediated knockdown induced by treatment with 2.5 mM glucosamine for 36 h (GlcN) compared to untreated control (Ctrl). Wunderlich J, Kotov V, Votborg-Novél L, Ntalla C, Geffken M, Peine S, Portugal S, Strauss J. Iron transport pathways in the human malaria parasite Plasmodium falciparum revealed by RNA-sequencing. Front Cell Infect Microbiol. 2024 14:1480076.