Effects of GAP50 binders on schizont egress and merozoite invasion (b) shows DAPI and immunofluorescence staining of 8 h test molecule treated segmental schizonts. Briefly, parasites were treated with test molecules selected from panel (a), followed by fixation and staining with DAPI (5 μg/mL) and 1 anti-Phil1 antibody raised in rabbit followed by staining with Alexa 488 fluorophore-conjugated anti-rabbit antibody. Notice the Phil1 staining inside the cell in control vs on the periphery in Bedaquiline and Hayatinine treated cultures and the remaining three schizontocidal molecules (Curine, Brilacidin, and MMV688271) exhibiting massive schizont staining source not available). Lindström M, Chen L, Jiang S, Zhang D, Gao Y, Zheng J, Hao X, Yang X, Kabbinale A, Thoma J, Metzger LC, Zhang DY, Zhu X, Liu H, Gustafsson CM, Burmann BM, Winderickx J, Sunnerhagen P, Liu B. Lsm7 phase-separated condensates trigger stress granule formation. Nat Commun. 2022 13(1):3701. PMID: 35764627