Comparison of the location of PfSec31p with the ER-located protein PfERC in P.-falciparum-infected erythrocytes and semiquantitative analysis of the amount of PfSec31p in the erythrocyte cytosol. (a-h) Asynchronous infected erythrocytes (K1 strain) were probed with affinity-purified rabbit anti-PfSec31(WD) antiserum followed by a fluorescein-conjugated anti-rabbit IgG (green fluorescence) and a murine antiserum recognising the ER located protein PfERC followed by Alexa-Fluor-568-conjugated antimouse IgG (red fluorescence). A region of partial overlap ofPfSec31p and PfERC in the parasite cytosol in (d) is marked with an arrowhead. (i-p) Asynchronous infected erythrocytes (K1 strain) were probed with rabbit anti-PfSec31(WD) antiserum (green fluorescence; i-l) or a murine anti-PfERC (red fluorescence; m-p). Four optical slices (0.6 mm) were collected by confocal microscopy from the bottom to the top of the parasitised erythrocytes.
Adisa A, Albano FR, Reeder J, Foley M, Tilley L. Evidence for a role for a Plasmodium falciparum homologue of Sec31p in the export of proteins to the surface of malaria parasite-infected erythrocytes. J Cell Sci. 2001 114(Pt 18):3377-86.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1108600 | endoplasmic reticulum-resident calcium binding protein |