PfRbsn5, PfVPS45, and PfRab5b inactivation-induced vesicles are hemoglobin filled and not connected to the host cell. Microscopy images of -hemoglobin (Hemoglobin) IFAs of formaldehyde-fixed, PfVPS45endo, PfRbsn5endo, and PfRab5bendo knock-sideways (6 h rapalog) and control parasites (cell lines indicated). White arrows: accumulated hemoglobin positive signals, blue arrows: DV. Nuclei were stained with DAPI. DIC, differential interference contrast.

Scale bar, 5 μm.

Sabitzki R, Roßmann AL, Schmitt M, Flemming S, Guillén-Samander A, Behrens HM, Jonscher E, Höhn K, Fröhlke U, Spielmann T. Role of Rabenosyn-5 and Rab5b in host cell cytosol uptake reveals conservation of endosomal transport in malaria parasites. PLoS Biol. 2024 22(5):e3002639. PMID: 38820535