Plasmodium falciparum export protein PFE60 is a resident Maurer’s cleft protein not exposed on the infected red blood cell surface. (B) Dual immunofluorescence assays of PFE60 with exported proteins localised in different areas of the infected red blood cell. CS2WT or PFE60-hemagglutinin/Strep infected red blood cells were probed with anti-PFE60 (or anti-hemagglutinin) (first column), and co-localisation studies carried out with anti-P. falciparum Exported Membrane Protein 3, anti-Plasmodium Transport Protein 2, anti-Heat Shock Protein 70x, anti-Plasmodium transport protein1 and antiskeleton-
binding protein 1 (second column). PFE60 strongly co-localises with the MC resident proteins skeleton-binding protein 1 and Plasmodium transport protein1. The ‘Overlay’ column shows an overlay of the two fluorescence signals and merged images of the fluorescence signal, DAPI and bright field are depicted in the ‘Merge’ column. Scale bar: 4 lm. (C) Trypsin treatment of live PFE60-GFP infected red blood cells (20–28 h post invasion). The same punctate GFP signal was observed in trypsin treated infected red blood cells as in untreated cells indicating that PFE60 is not exposed on the surface of infected red blood cells. (- tryp) untreated cells; (+ tryp) trypsin treated cells; (+ tryp/PMSF) cells treated with trypsin plus protease inhibitor phenylmethane sulfonyl fluoride. The ‘PFE60-GFP’ column shows the fluorescence signal and the ‘Merge’
column shows the overlay of the fluorescence signal and the bright field image. Scale bar: 4 mm
Chang M, Faou P, Maier AG, Rug M. Plasmodium falciparum export protein PFE60 influences Maurer's cleft architecture and virulence complex composition. Int J Parasitol. 2017 Oct pii: S0020-7519(17)30302-8.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0201900 | erythrocyte membrane protein 3 |
PF3D7_0202200 | EMP1-trafficking protein |
PF3D7_0501200 | parasite-infected erythrocyte surface protein |
PF3D7_0501300 | skeleton-binding protein 1 |
PF3D7_0731100 | gametocyte exported protein 11 EMP1-trafficking protein |