Mutant F-P2 has a microneme secretion defect. IFA of Mic2 and IMC3 (marking the peripheral cytoskeleton) of wild-type and F-P2 at 40°C with or without ionophore stimulation shows micronemes are intact in F-P2. Phase images show vacuolar membrane is intact in F-P2 at 40°C. Asterisk marks egressing parasite. Andrews M, Baum J, Gilson PR, Wilson DW. Bottoms up! Malaria parasite invasion the right way around. Trends Parasitol. 2023 39(12):1004-1013.