PfAMA1 translocation is aberrant in schizonts with PfMOP-knockdown. (a) Schizonts from [+]/[-] Shld1 PfMOP-DD parasites were E64-treated, fixed, probed with anti-PfAMA1 and scored as micronemal (M), partially translocated (PT) or surface (S), representative [+] Shld1 parasite IFAs shown. (c) Synchronized schizont stage (40–44 h) parasites, maintained with 250nM (left panel) or 0 nM (right panel) Shld1, were incubated 6 h in presence of 10 mM E64, methanol-fixed, permeabilized, and stained using antibodies against PfRON4, PfRhopH3, PfEBA175 and PfTubulin. Staining for these markers was similar in [+] and [-] Shld1 conditions. PfAMA1 staining was used to identify E64-treated schizonts that were sufficiently mature (that is, surface staining or partially translocated staining, but not micronemal staining, scale bar, 1 mm).
Absalon S, Robbins JA, Dvorin JD. An essential malaria protein defines the architecture of blood-stage and transmission-stage parasites. Nat Commun. 2016 7:11449.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0731500 | erythrocyte binding antigen-175 |
PF3D7_0903700 | alpha tubulin 1 |
PF3D7_0917000 | condensin complex subunit 3, putative? cnd3 |
PF3D7_1116000 | rhoptry neck protein 4 |
PF3D7_1133400 | apical membrane antigen 1 |
PF3D7_1469600 | acetyl-coa carboxylase |