Immunolocalisation of putative membrane proteins in Plasmodium. (a) Plasmodium berghei and Plasmodium falciparum (PfDMT2‐glmS clone D6, PfABCB4‐HA clone G3, and PfABCB4‐glmS clone F11) candidate proteins were HA‐tagged and probed with rat anti‐HA primary and anti‐rat 488 secondary antibodies (green). The apicoplast was probed with rabbit anti‐ACP primary and anti‐rabbit 546 secondary antibodies (red) and nuclei were stained with Hoechst (blue). Channels were merged with a transmission image (right) showing the location within the parasite. All candidates were localised within red blood cell stage parasites. Localisation of PfDMT2‐glmS, PfABCB4‐glmS, and PfABCB4‐HA confirmed the predicted apicoplast localisations and that the HA‐tag and glmS additions do not interfere with targeting. (b) Pf3D7_1145500‐GFP was localised in an intraerythrocytic parasite probed with mouse anti‐GFP primary and anti‐mouse 488 secondary antibodies (green; scale bar is 2 μm).
Sayers CP, Mollard V, Buchanan HD, McFadden GI, Goodman CD. A genetic screen in rodent malaria parasites identifies five new apicoplast putative membrane transporters, one of which is essential in human malaria parasites. Cell Microbiol. 2018 Jan;20(1).
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_0208500 | acyl carrier protein |
PF3D7_0302600 | ABC transporter B family member 4, putative, ABCB4 |