DRM association is necessary but not sufficient to target host proteins to the malarial vacuolar membrane. Normal human erythrocytes were infected with asexual stage P. falciparum. Infected cultures were sampled and prepared for indirect immunofluorescence assays. Cells were adhered to 0.1%poly-Llysine–coated glass coverslips, fixed, washed, and permeabilized before treatments with appropriate primary and fluorescein-or rhodamine-conjugated secondary antibodies. Hoechst stain was used to visualize parasite nuclei. Fluorescence microscopy images were obtained using DeltaVision deconvolution. 1 Images are single optical sections of cells infected with ring-stage parasites. Scale bars present size (in m). All images were obtained with a 100 objective. Murphy SC, Samuel BU, Harrison T, Speicher KD, Speicher DW, Reid ME, Prohaska R, Low PS, Tanner MJ, Mohandas N, Haldar K. Erythrocyte detergent-resistant membrane proteins: their characterization and selective uptake during malarial infection. Blood. 2004 103(5):1920-8. PMID: 14592818.