Kelch13 location and interactome. (Left panel) Fluorescence microscopy images showing parasites with endogenously GFP-tagged Kelch13 (K13) (17) with coexpressed STVR-SDEL (ER), Sec13p (ER exit sites), P40 (phosphatidylinositol 3-phosphate sensor: food vacuole), ACP (apicoplast), or endogenously GFP-tagged mutated Kelch13 (K13C580Y) with episomal mCherry-Kelch13wt.
(Right panel) Fluorescence microscopy in parasites with endogenously GFP-tagged DiQ-BioID hits (KIC1-10 or short name) colocalized with episomal mCherry-Kelch13. Arrowheads indicate Kelch13 foci overlapping with the KIC signal. Scale bars, 5 mm. Merge, merged green and red channel; DAPI, 4′,6-diamidino-2-phenylindole (nuclei).
Birnbaum J, Scharf S, Schmidt S, Jonscher E, Hoeijmakers WAM, Flemming S, Toenhake CG, Schmitt M, Sabitzki R, Bergmann B, Fröhlke U, Mesén-Ramírez P, Blancke Soares A, Herrmann H, Bártfai R, Spielmann T. A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites. Science. 2020 367(6473):51-59. PMID: 31896710.
Other associated proteins
| PFID | Formal Annotation |
|---|---|
| PF3D7_0104300 | ubiquitin carboxyl-terminal hydrolase 1, putative |
| PF3D7_0606000 | kelch13 interaction candidate kic1 |
| PF3D7_0609600 | probable protein, unknown function |
| PF3D7_0914400 | kelch13 interaction candidate kic3 |
| PF3D7_0915400 | ATP-dependent 6-phosphofructokinase |
| PF3D7_1014900 | kelch13 interaction candidate kic8 |
| PF3D7_1017300 | golgi re-assembly stacking protein 2 |
| PF3D7_1122500 | kelch13 interaction candidate kic10 |
| PF3D7_1138700 | kelch13 interaction candidate kic5 |
| PF3D7_1227700 | kelch13 interaction candidate kic2 |
| PF3D7_1230700 | protein transport protein SEC13 |
| PF3D7_1246300 | kelch13 interaction candidate kic4 |
| PF3D7_1322000 | nucleoside-diphosphatase, putative |
| PF3D7_1323000 | beta-hydroxyacyl-ACP dehydratase |
| PF3D7_1343700 | kelch protein K13 |
| PF3D7_1442400 | kelch13 interaction candidate kic9 |
| PF3D7_1459100 | GTP-binding protein, putative |
