Subcellular locations of PfAK2C4A/GFP in which the cysteine at position 4 of AK2 has been replaced with alanine, and PfAK2/GFP in which the glycine (position 2) and cysteine (position 4) residues were each replaced with alanine. (A) The AK2C4A/GFP and AK2G2AC4A/GFP proteins, respectively, were expressed using the CRT promoter (construct indicated above the images). The intracellular location of the AK2C4A/GFP protein detected by epifluorescence microscopy in early and late trophozoite stages showed some localised patch-like structures within the parasite, a different pattern from the signal seen for PfAK2/GFP or PfAK2G2A/GFP. The intracellular location of the AK2G2AC4A/GFP was uniform throughout the parasite cytoplasm, and reminiscent of the distribution of PfAK2G2A/GFP.. Thavayogarajah T, Gangopadhyay P, Rahlfs S, Becker K, Lingelbach K, Przyborski JM, Holder AA. Alternative Protein Secretion in the Malaria Parasite Plasmodium falciparum. PLoS One. 2015 Apr 24;10(4):e0125191. PMID: 25909331