Upper panel: PfJ2 is an essential, ER-resident protein. A) PfJ2apt parasites were fixed with paraformaldehyde and glutaraldehyde and stained with DAPI (blue) and with antibodies against HA (green) and the ER-marker PfPMV (red). Ring (R), Trophozoite (T), and Schizont (S) stage parasites are shown. Z-stack Images were deconvoluted and shown as a single, maximum intensity projection. Scale bar represents 5 μm. Lower panel: PfPDI8 and PfPDI11 are redox-active ER proteins. PfJ2apt-PDI8glmS and PfJ2apt-PDI11glmS parasites were fixed with paraformaldehyde and glutaraldehyde and stained with DAPI (blue) and with antibodies against HA (green) and V5 (red). Ring (R), Trophozoite (T), and Schizont (S) stage parasites are shown. Z-stack Images were deconvoluted and shown as a single, maximum intensity projection. Scale bar represents 5 μm.
Cobb DW, Kudyba HM, Villegas A, Hoopmann MR, Baptista RP, Bruton B, Krakowiak M, Moritz RL, Muralidharan V. A redox-active crosslinker reveals an essential and inhibitable oxidative folding network in the endoplasmic reticulum of malaria parasites. PLoS Pathog. 2021 17(2):e1009293.
Other associated proteins
PFID | Formal Annotation |
---|---|
PF3D7_1108700 | heat shock protein 40, type ii |
PF3D7_1134100 | protein disulfide isomerase, putative |
PF3D7_1323500 | PEXEL protease plasmepsin V |