Identification of PhIL1-interacting candidates using BioID. (a) IMC localisation of PfPhIL1-BirA-GFP in schizonts and merozoites. Nuclei were stained with Hoechst-33,342. Scale bar, 2 μm. (b) PfPhIL1-based biotinylation at the IMC. PfPhIL1-BirA-GFP (mouse anti-GFP, green)or 3D7 control parasites were fixed with MeOH at the schizont stage and biotinylated proteins were visualized by streptavidin-594 (red).Hoechst was used for DNA staining (blue). Scale bar, 1 μm. Wichers JS, Wunderlich J, Heincke D, Pazicky S, Strauss J, Schmitt M, Kimmel J, Wilcke L, Scharf S, von Thien H, Burda PC, Spielmann T, Löw C, Filarsky M, Bachmann A, Gilberger TW. Identification of novel inner membrane complex and apical annuli proteins of the malaria parasite Plasmodium falciparum. Cell Microbiol. 2021 23(9):e13341.