PF3D7_0831800 histidine-rich protein II

The Maurer’s clefts are intermediates in PfHRPII transport and are targeted by its histidine-rich sequences. Single optical sections of trophozoite infected cells expressing SSGFPHis154–327 (A–C) or PfHRPIIGFP (D–F) are shown. Cells were fixed, permeabilized, subjected to indirect immunofluorescence assays and probed with antibodies to GFP (green) and a Maurer’s cleft resident protein PfSBP (red). Magnification of the boxes in C and F are shown as C’ and F’, respectively. The extent of colocalization is shown in yellow. In C’ and F’, arrows indicate GFP-labeled tubules or vesicles connecting clefts (arrowheads) to each other or the vacuolar parasite (P). Nuclei were stained with Hoechst (blue). (Scale bar, 5 mm.)

Lopez-Estraño C, Bhattacharjee S, Harrison T, Haldar K. Cooperative domains define a unique host cell-targeting signal in Plasmodium falciparum-infected erythrocytes. Proc Natl Acad Sci U S A. 2003 100:12402-7. Copyright (2009) National Academy of Sciences, U.S.A

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